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KMID : 1094720080130060666
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Biotechnology and Bioprocess Engineering
2008 Volume.13 No. 6 p.666 ~ p.670
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Optimization of culture conditions for 1,3-propanediol production from crude glycerol by Klebsiella pneumoniae using response surface methodology
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Oh Baek-Rock
Seo Jeong-Woo
Choi Min-Ho
Kim Chul-Ho
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Abstract
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To produce 1,3-propanediol (1,3-PD) from crude glycerol, cultivation conditions were optimized by response surface methodology (RSM) based on a 25 factorial central composite design (CCD). RSM was adopted to derive a statistical model for the individual and interactive effects of crude glycerol, (NH4)2SO4, pH, cultivation time and temperature on the production of 1,3-PD. Optimal conditions for maximum 1,3-PD production were as follows: crude glycerol, 35 g/L; (NH4)2SO4, 8 g/L; pH, 7.37; cultivation time, 10.8 h; temperature, 36.88°C. Under these optimal conditions, the design expert presented the maximal numerical solution with a predicted 1,3-PD production level of up to 13.74 g/L. The experimental production of 1,3-PD yielded 13.8 g/L, which was in close agreement with the model prediction.
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KEYWORD
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central composite design, crude glycerol, 1, 3-propanediol, Klebsiella pneumoniae, response surface methodology
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